In a special conference to commemorate the anniversary, leaders from the Church of Ireland, the Methodist and Presbyterian Churches in Ireland, the Catholic Church, and the Irish Council of Churches will participate and share their personal perspectives.
The UK and Ireland's official representatives to the Vatican City State, the British and Irish ambassadors to the Holy See, coordinated the event jointly.
The embassies previously held a comparable ceremony for the 20th anniversary in 2018.
The conference, which takes place at the Pontifical Irish College, will also look at how churches and other leaders in the religious community generally contribute to world peace and harmony.
Speaking in advance of the trip, the British ambassador to the Holy See, Chris Trott, and the Irish ambassador, Frances Collins, expressed the hope that by exchanging personal stories, the visit would serve as an example for other Church and religious leaders who are working to promote peace and reconciliation in the world.
The church leaders expressed their appreciation for the chance to pause, think back on the previous 25 years, and look forward to the future with "a common allegiance to Jesus as Lord" in a joint statement.
"We are grateful to everyone who contributed to the Belfast/Good Friday Agreement's success. We are also conscious of all those who have endured and still endure suffering as a result of pointless violence over the years," they added.
The Church Leaders Group includes Bishop Andrew Forster of the Irish Council of Churches, Archbishop Eamon Martin of the Catholic Primate of All Ireland, Archbishop John McDowell of the Church of Ireland Primate of All Ireland, Rev. Sam Mawhinney, Moderator of the General Assembly of the Presbyterian Church in Ireland, and Rev. David Turtle, President of the Methodist Church in Ireland.
Due to the urgency of the political developments at the time, the group was founded in the 1960s, and since then, they have met on a regular basis.
In a statement, Ambassadors Frances Collins and Chris Trott emphasised the crucial part that churches have played in promoting peace and reconciliation, including laying the groundwork for the agreement's successful negotiation, fostering intercommunity dialogue, and encouraging "a deeper understanding of the root causes of the conflict" through their ecumenical work.
They added that "their invaluable work continues today, as they support and mentor communities throughout Northern Ireland as they confront the extremely difficult challenges of surviving in a post-conflict society."